New data on pollen morphology of the genus Camphorosma (Chenopodiaceae)
More details
Hide details
Department of Systematics and Floristics of Vascular Plants, M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, 2, Tereshchenkivska St, Kyiv 01004, Ukraine
Online publication date: 2023-04-28
Publication date: 2023-04-28
  • Pollen morphology of Camphorosma species was studied by LM and SEM.
  • Features of Camphorosma pollen grains diagnostic at species level were revealed.
  • Cluster analysis mainly supports the definition of pollen species.
  • Molecular phylogenetic data for four species are supported by the palynomorphology.
Recent molecular phylogenetic studies of Camphorosmoideae (Chenopodiaceae) demonstrated that Camphorosma is monophyletic. Palynomorphological peculiarities are used in taxonomy as additional diagnostic features and pollen analysis. Pollen morphology was studied using light and scanning electron microscopy, based on 13 specimens belonging to four species (C. annua, C. lessingii, C. monspeliaca and C. songorica). The aim of this research was to provide new data on pollen characteristics of the above mentioned species and to evaluate their taxonomic value. The results obtained show that pollen grains are pantoporate, spheroidal, circular in outline, undulate or slightly undulate on the edges; small or medium-sized. Pores are circular, with distinct margins. Exine sculpture is nanoechinate, tectum psilate or psilate-perforate. Pore membranes are nanoechinate. Taxonomic relevance of the most important characters of pollen grains (pollen diameter, pore number, pore diameter, distance between pores and between pore centres, C/D value, nanoechini density, presence/absence of perforations, number of nanoechini on pore membranes, structure of columellae) is discussed. The exine structure of pollen grains of all species was analysed for the first time in the current study. Pollen morphology proved to be an important additional source of information for taxonomy for species-specific identification and pollen analysis within Camphorosma. Palynomorphological data are interpreted in the existing phylogenetic framework.
Albach, D.C., Tsymbalyuk, Z.M., Mosyakin, S.L., 2021. Pollen morphology of Ellisiophyllum and Sibthorpia (Plantaginaceae, tribe Sibthorpieae) and phylogenetics of the tribe. Plant Systematics and Evolution 307, article 66. https://doi.org/10.1007/s00606....
Bezusko, L.G., Mosyakin, S.L., Tsymbalyuk, Z.M., 2003. Pollen of the family Chenopodiaceae Vent. as an indicator of natural and anthropogenic changes of vegetation of Ukraine in the Holocene. Naukovi zapysky NaUKMA. Pryrodnychi Nauky 22(3), 392–395 (in Ukrainian).
Bezusko, L.G., Mosyakin, S.L., Tsymbalyuk, Z.M., 2006. Pollen of Chenopodiaceae Vent. as an indicator of changes of natural conditions in Ukraine in the Pleistocene. Ukrainian Botanical Journal 63(5), 645–653 (in Ukrainian).
Bezusko, L.G., Mosyakin, S.L., Tsymbalyuk, Z.M., 2019. Spatio-temporal differentiation of distribution patterns of Salicornia perennans, Halimione verrucifera, and Suaeda cf. prostrata (Chenopodiaceae) in the plain part of Ukraine during the Allerød-Holocene. Journal of Geography and Geoecology 28(2), 221–229.
Brummitt, R.K., Powell, C.E., (eds), 1992. Authors of plant names. A list of authors of scientific names of plants, with recommended standard forms of their names, including abbreviations. Royal Botanic Gardens, Kew.
El Ghazali, G.E.B., 2022. Pollen morphological studies in Amaranthaceae s. lat. (incl. Chenopodiaceae) and their taxonomic significance: A review. Grana 61(1), 1–7. https://doi.org/10.1080/001731....
Erdtman, G., 1952. Pollen morphology and plant taxonomy. Angiosperms. Stockholm. Almqvist & Wiksell.
Freitag, H., Kadereit, G., 2014. C3 and C4 leaf anatomy types in Camphorosmeae (Camphorosmoideae, Chenopodiaceae). Plant Systematics and Evolution 300, 665–687. https://doi.org/10.1007/s00606....
Halbritter, H., Ulrich, S., Grímsson, F., Weber, M., Zetter, R., Hesse, M., Buchner, R., Svojtka, M., Frosch-Radivo, A., 2018. Illustrated pollen terminology. Cham, Switzerland. Springer International Publishing. 2nd ed. http://doi.org/10.1007/978-3-3....
Hammer, Ø., Harper, D.A.T., Ryan, P.D., 2001. Past: paleontological statistics software package for education and data analysis. Palaeontologica Electronica 4(1), article 4.
Ianovici, N., 2008. Seasonal variations in the atmospheric Chenopodiaceae/Amaranthaceae pollen count in Timisoara, Romania. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 36(2), 17–22. https://doi.org/10.15835/nbha3....
Iljin, M.M., 1936. Camphorosma. In: Komarov, V.L., Shishkin, B.K. (eds), Flora of the USSR [Flora SSSR], vol. 6. Moscow–Leningrad, USSR. Izdatelstvo AN USSR, pp. 10–91 (in Russian).
Iljin, M.M., 1952. Camphorosma. In: Kotov, M.I. (ed.), Flora of the Ukrainian SSR [Flora URSR], vol. 4. Kyiv, Ukraine. Vydavnytstvo AN URSR, pp. 344–347 (in Ukrainian).
IPNI, International Plant Names Index, 2023. The Royal Botanic Gardens, Kew, Harvard University Herbaria & Libraries and Australian National Botanic Gardens. Available from: http://www.ipni.org. Accessed 18 January 2023.
Kadereit, G., Freitag, H., 2011. Molecular phylogeny of Camphorosmeae (Camphorosmoideae, Chenopodiaceae): Implications for biogeography, evolution of C4 – photosynthesis and taxonomy. Taxon 60(1), 51–78. https://doi.org/10.1002/tax.60....
Kadereit, G., Lauterbach, M., Pirie, M.D., Arafeh, R., Freitag, H., 2014. When do different C4 leaf anatomies indicate independent C4 origins? Parallel evolution of C4 leaf types in Camphorosmeae (Chenopodiaceae). Journal of Experimental Botany 65(13), 3499–3511. https://doi.org/10.1093/jxb/er....
Kupriyanova, L.A., Alyoshina, L.A., 1972. Pyltsa i spory rasteniy flory evropeyskoy chasti SSSR, vol. 1. Leningrad. Nauka (in Russian).
Kurşat, M., Özdağ, N., Başer, B., Özbey, F., Emre, I., 2022. A palynological investigation of some taxa of the genus Ranunculus L. (Ranunculaceae) in Turkey and its taxonomic value. Palynology 46(4), 1–13. https://doi.org/10.1080/019161....
Lu, K.Q., Li, M., Wang, G.H., Xu, L.S., Ferguson, D.K., Trivedi, A., Xuan, J., Feng, Y., Li, J.F., Xie, G., Yao, Y.F., Wang, Y.F., 2018. New pollen classification of Chenopodiaceae for exploring and tracing desert vegetation evolution in eastern arid central Asia. Journal of Systematics and Evolution 57(2), 190–199. https://doi.org/10.1111/jse.12....
McAndrews, J.H., Swanson, A.R., 1967. The pore number of periporate pollen with special references to Chenopodium. Review of Palaeobotany and Palynology 3, 105–117.
Monoszon, M.Kh., 1973. Opredelitel pyltsy vidov semeystva marevykh (posobie po sporovo-pyltsevomu analizu). Moscow. Nauka (in Russian).
Mosyakin, S.L., 1996. Camphorosma. In: Tzvelev, N.N. (ed.), Flora Europae Orientalis, vol. 9. Petropoli. Mir i Semia–XCV, pp. 58–59 (in Russian).
Mosyakin, S.L., Fedoronchuk, M.M., 1999. Vascular plants of Ukraine. A nomenclatural checklist. Kyiv. M.G. Kholodny Institute of Botany.
Mosyakin, S.L., Tsymbalyuk, Z.M., 2015. Pollen morphology of the tribes Aptosimeae and Myoporeae supports the phylogenetic pattern in early-branching Scrophulariaceae revealed by molecular studies. Willdenowia 45(2), 209–222. https://doi.org/10.3372/wi.45.....
Mosyakin, S.L., Tsymbalyuk, Z.M., 2017. Pollen morphology of the tribe Hemimerideae: possible evidence of ancestral pollen types and parallel evolution in the basalmost clade of Scrophulariaceae s. str. Willdenowia 47(1), 15–27. https://doi.org/10.3372/wi.47.....
Nowicke, J.W., 1975. Pollen morphology in the order Centrospermae. Grana 15, 51–77.
Perveen, A., Qaiser, M., 2012. Pollen flora of Pakistan: Chenopodiaceae. Pakistan Journal of Botany 44(4), 1325–1333.
Piotrowska-Weryszko, K., Weryszko-Chmielewska, E., Sulborska, A., Puc, M., Malkiewicz, M., Siergiejko, Z., Dąbrowska-Zapart, K., Ziemianin, M., Rapiejko, A., Wieczorkiewicz, A., Jurkiewicz, D., Kalinowska, E., Lipiec, A., 2020. Concentration of pollen of Chenopodiaceae/Amaranthaceae plants in the air of selected Polish cities in 2020. Alergoprofil 16(4), 34–40. http://doi.org/10.24292/10.242....
POWO, Plants of the World Online, 2023. Facilitated by the Royal Botanic Gardens, Kew. Available from: http://www.plantsoftheworldonl.... Accessed 18 January 2023.
Punt, W., Hoen, P.P., Blackmore, S., Nilsson, S., Le Thomas, A., 2007. Glossary of pollen and spore terminology. Review of Palaeobotany and Palynology 143(1–2), 1–81. https://doi.org/10.1016/j.revp....
Savitsky, V.D., Bezusko, L.G., Butich, N.G., Tsymbaliuk, Z.M., Savitska, O.V. and Bezusko, T.V., 1996. Airborne pollen in Kiev (Ukraine): gravimetric sampling. Aerobiologia 12, 209–211.
Scott, A.J., 1978. A revision of the Camphorosmioideae (Chenopodiaceae). Feddes Repertorium 89(2–3), 101–119. https://doi.org/10.1002/fedr.1....
Sonyan, H., Hayrapetyan, A., Poghosyan, N., Muradyan A., 2022. Pollen typification and palynotaxonomy of subfamily Salsoloideae Ulbr. (Chenopodiaceae Vent.) in the flora of South Transcaucasia. Acta Palaeobotanica 62(2), 93–107. https://doi.org/10.35535/acpa-....
Tashev, A., Dimitrova, V., 2019. Medicinal Plants of Bulgaria. Current Perspectives on Medicinal and Aromatic Plants 2(1), 29–39. https://doi.org/10.38093/cupma....
Thiers, B., 2023 (continuously updated). Index Herbariorum: A global directory of public herbaria and associated staff. New York Botanical Garden’s Virtual Herbarium. Available from: http://sweetgum.nybg.org/scien.... Accessed 18 January 2023.
Tsymbalyuk, Z.M., 2005. Pollen morphology of representatives of the family Chenopodiaceae Vent. (for taxonomy and spore-pollen analysis). Ph.D. Thesis, M.G. Kholodny Institute of Botany, NAS of Ukraine. Kyiv, Ukraine (in Ukrainian).
Tsymbalyuk, Z.M., 2008. Palynomorphological peculiarities of the family Chenopodiaceae. Botanicheskii Zhurnal 93(3), 430–438 (in Russian).
Tsymbalyuk, Z.M., Mosyakin, S.L., Bezusko, L.G., 2005. New approaches to developing an identification manual of pollen of Chenopodiaceae for pollen analysis (taxa occurring in Ukraine). Naukovi zapysky NaUKMA. Biolohiya ta Ekolohiya 43, 19–25 (in Ukrainian).
Tsymbalyuk, Z.M., Bezusko, L.G., Nitsenko, L.M., 2021a. Palynotheca of the M.G. Kholodny Institute of Botany, NAS of Ukraine. In: Mosyakin, S.L. et al. (eds), Herbaria of the 21st century: Achievements and challenges: Proceedings of International Scientific Conference dedicated to the 100th anniversary of the National Herbarium of Ukraine (KW). M.G. Kholodny Institute of Botany. Kyiv, pp. 38–41 (in Ukrainian).
Tsymbalyuk, Z.M., Celenk, S., Mosyakin, S.L., Nitsenko, L.M., 2021b. Pollen morphology of some species of the genus Cephalaria Schrad. (Caprifoliaceae) and its significance for taxonomy. Microscopy Research and Technique 84(4), 682–694. http://dx.doi.org/10.1002/jemt....
Tsymbalyuk, Z.M., Ivanova, D., Nitsenko, L.M., 2021c. Taxonomic significance of palynomorphological characteristics of selected Centranthus (Caprifoliaceae) species. Hacquetia 20(2), 243–256. http://dx.doi.org/10.2478/hacq....
Tsymbalyuk, Z.M., Celenk, S., Bell, C.D., Nitsenko, L.M., Mosyakin, S.L., 2022a. Comparative palynomorphological study of the genus Symphoricarpos (Caprifoliaceae): exine sculpture and implications for evolution. Palynology 46(4), 1–14. https://doi.org/10.1080/019161....
Tsymbalyuk, Z.M., Ivanova, D., Nitsenko, L.M., 2022b. Taxonomic significance of pollen morphology of Pterocephalus plumosus (Caprifoliaceae). Botanica Serbica 46(2), 229–238. https://doi.org/10.2298/BOTSER....
Tsymbalyuk, Z.M., Nitsenko, L.M., Mosyakin, S.L., 2022c. The unique type of pollen grain of Dasistoma (Macranthera-Agalinis clade; Orobanchaceae): implications for taxonomy. Grana 61(6), 436–447. https://doi.org/10.1080/001731....